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Literature summary extracted from

  • Kyossev, Z.; Weigel, P.H.
    An enzyme capture assay for analysis of active hyaluronan synthases (2007), Anal. Biochem., 371, 62-70.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.212 Homo sapiens hyaluronan synthase 1 is cloned into the plasmid pFLAG-CMV2, for in vitro translation the pF3A WG(BYDV) Flexi vector is used Homo sapiens
2.4.1.212 Streptococcus equisimilis hyaluronan synthase is cloned into the plasmid pKK223-3 for expression in Escherichia coli SURE cells Streptococcus dysgalactiae subsp. equisimilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.212 UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] Streptococcus dysgalactiae subsp. equisimilis
-
 UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan]
-
 ?
2.4.1.212 UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan] Homo sapiens
-
 UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan]
-
 ?
2.4.1.212 UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] Streptococcus dysgalactiae subsp. equisimilis
-
 UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan]
-
 ?
2.4.1.212 UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan] Homo sapiens
-
 UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.212 Homo sapiens
-
-
-
2.4.1.212 Streptococcus dysgalactiae subsp. equisimilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.212 on a Ni2+-nitrilotriacetic acid resin Streptococcus dysgalactiae subsp. equisimilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.212 UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan]
-
 Streptococcus dysgalactiae subsp. equisimilis UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan]
-
 ?
2.4.1.212 UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan]
-
 Homo sapiens UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-[nascent hyaluronan]
-
 ?
2.4.1.212 UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan]
-
 Streptococcus dysgalactiae subsp. equisimilis UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan]
-
 ?
2.4.1.212 UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan]
-
 Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-(1-4)-beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-glucosaminyl-(1-4)-[nascent hyaluronan]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.212 HAS
-
 Streptococcus dysgalactiae subsp. equisimilis
2.4.1.212 HAS
-
 Homo sapiens
2.4.1.212 HAS1
-
 Homo sapiens
2.4.1.212 HsHAS1
-
 Homo sapiens
2.4.1.212 hyaluronan synthase
-
 Streptococcus dysgalactiae subsp. equisimilis
2.4.1.212 hyaluronan synthase
-
 Homo sapiens
2.4.1.212 seHAS
-
 Streptococcus dysgalactiae subsp. equisimilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.212 22
-
 HAS capture assay, overnight at room temperature Streptococcus dysgalactiae subsp. equisimilis
2.4.1.212 22
-
 HAS capture assay, overnight at room temperature Homo sapiens
2.4.1.212 30
-
 standard assay for HAS activity Streptococcus dysgalactiae subsp. equisimilis
2.4.1.212 37
-
 standard assay for HAS activity Homo sapiens
2.4.1.212 60
-
 HAS capture assay, 3 h Streptococcus dysgalactiae subsp. equisimilis
2.4.1.212 60
-
 HAS capture assay, 3 h Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.212 5
-
 HAS capture assay Streptococcus dysgalactiae subsp. equisimilis
2.4.1.212 5
-
 HAS capture assay Homo sapiens